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Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

DOI :

10.3791/50308-v

13:32 min

April 10th, 2013

April 10th, 2013

20,814 Views

1Department of Anesthesiology, University of Texas Medical Branch

We describe how to use laser capture microdissection (LCM) to obtain enriched populations of hippocampal neurons or single neurons from frozen sections of the injured rat brain for subsequent gene expression analysis using quantitative real time PCR and/or whole-genome microarrays.

Tags

Laser Capture Microdissection

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