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Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

5.1K Views

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09:54 min

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March 8th, 2020

DOI :

10.3791/60612-v

March 8th, 2020

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5,125 Views

Sean J. O'Sullivan¹^,², Beverly A.S. Reyes³, Rajanikanth Vadigepalli¹, Elisabeth J. Van Bockstaele³, James S. Schwaber¹

¹Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, ²Sidney Kimmel Medical College, Thomas Jefferson University, ³Department of Pharmacology & Physiology, Drexel University College of Medicine

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Summary

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Laser Capture Microdissection

Microfluidic QPCR

Transcriptional Profiles

Systems Biology

Opioid Dependence

Gene Expression

Cellular Subphenotypes

Anatomic Specificity

Biological Problems

Ethanol Dehydration

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