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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

DOI :

10.3791/1350-v

August 14th, 2009

August 14th, 2009

51,921 Views

1Protein Expression and Purification Core Facility, EMBL Heidelberg

A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.

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Proteins

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