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Chromatographic Purification of Highly Active Yeast Ribosomes

DOI :

10.3791/3214-v

October 24th, 2011

October 24th, 2011

16,432 Views

1Department of Cell Biology and Molecular Genetics, University of Maryland , 2Department of Biotechnology and Microbiology, Vilnius University

Contamination of preparations of eukaryotic ribosomes purified by traditional methods by co-purifying nucleases and proteases negatively impacts on downstream biochemical and structural analyses. A rapid and simple chromatographic purification method is used to solve this problem using yeast ribosomes as a model system.

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Chromatographic Purification

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