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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

DOI :

10.3791/50354-v

September 10th, 2013

September 10th, 2013

22,116 Views

1Biochemistry and Molecular Biology, Colorado State University

A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.

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