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Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

DOI :

10.3791/62113-v

December 26th, 2020

December 26th, 2020

3,715 Views

1Department of Chemistry, Texas A&M University

Here we present a method to express acetylated histone proteins using genetic code expansion and assemble reconstituted nucleosomes in vitro.

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