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Chemistry

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Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples

13.9K Views

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06:57 min

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October 14th, 2013

DOI :

10.3791/50407-v

October 14th, 2013

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Diego Fajardo¹, Sastry S. Jayanty², Shelley H. Jansky¹

¹USDA-ARS and Department of Horticulture, University of Wisconsin - Madison, ²Department of Horticulture and Landscape Architecture, Colorado State University

Summary

This protocol describes a high through put colorimetric method that relies on the formation of a complex between iodine and chains of glucose molecules in starch. Iodine forms complexes with both amylose and long chains within amylopectin. After the addition of iodine to a starch sample, the maximum absorption of amylose and amylopectin occurs at 620 and 550 nm, respectively. The amylose/amylopectin ratio can be estimated from the ratio of the 620 and 550 nm absorbance values and comparing them to a standard curve in whi

Explore More Videos

Amylose Determination

Freeze Dried Potato Tuber

High Throughput

Colorimetric Method

Iodine starch Complex

Chapters in this video

0:01

Title

1:07

Amylose determination from potato tuber starch

5:39

Tables and Figures

6:31

Conclusions

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