Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
11.7K Views
•
16:19 min
•
September 10th, 2013
DOI :
September 10th, 2013
•Lorenzo Albertazzi1, Barbara Storti2, Marco Brondi2, Sebastian Sulis Sato2, Gian Michele Ratto3, Giovanni Signore4, Fabio Beltram2
1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST, Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST, Center for Nanotechnology Innovation
Chapters in this video
0:05
Title
2:30
Synthesis of the Sensors
4:20
In vitro pH Measurements
5:36
Cell Culture and Electroporation
6:38
pH Sensing in Living HeLa Cells
8:47
In vivo Sample Preparation
10:30
pH Imaging in Mouse Brain
12:24
Representative Analysis of Dendrimer-based pH Sensor Fluorescence
15:45
Conclusion
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