Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
34.3K Views
•
11:12 min
•
March 9th, 2013
DOI :
March 9th, 2013
•Brian C. Evans*1,2, Christopher E. Nelson*1,2, Shann S. Yu*1,2, Kelsey R. Beavers2,3, Arnold J. Kim1, Hongmei Li1,2, Heather M. Nelson4, Todd D. Giorgio1,2,5,6, Craig L. Duvall1,2
1Department of Biomedical Engineering, Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering, Vanderbilt University, 3Interdisciplinary Materials Science Program, Vanderbilt University, 4Monroe Carell Jr. Children's Hospital, Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering, Vanderbilt University, 6Department of Cancer Biology, Vanderbilt University
Chapters in this video
0:01
Title
0:26
Introduction fo Endosomolysis and Hemolysis
2:16
Preparation of Buffers and Test Reagents
3:14
Blood Sample Preparation
6:05
Assay Setup and Quantification
8:39
Data Analysis
9:00
Representative Results
9:25
Discussion
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