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Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

DOI :

10.3791/50548-v

August 31st, 2013

August 31st, 2013

17,619 Views

1School of Biochemistry, University of Bristol, 2Douglas Instruments

Here we describe a general method for random microseed matrix screening. This technique is shown to significantly increase the success rate of protein crystallization screening experiments, reduce the need for optimization, and provide a reliable supply of crystals for data collection and ligand-soaking experiments.

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Protein Crystallization

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