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Humanized Mediator Release Assay as a Read-Out for Allergen Potency

DOI :

10.3791/62702-v

10:22 min

June 29th, 2021

June 29th, 2021

2,859 Views

1Department of Biosciences, University of Salzburg

Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.

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Humanized Mediator Release Assay

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