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Humanized Mediator Release Assay as a Read-Out for Allergen Potency

3.5K Views

10:22 min

June 29th, 2021

DOI :

10.3791/62702-v

June 29th, 2021


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Humanized Mediator Release Assay

Chapters in this video

0:04

Introduction

1:04

Complement Inactivation of Human Sera

2:05

Humanized-RBL Cell Culture

4:07

Passive Sensitization of Humanized-RBL Cells

4:57

Antigen-stimulated Degranulation and Mediator Release

6:19

β-hexosaminidase Activity Measurement

7:21

Results: Effect of Allergen on β-hexosaminidase Release

9:38

Conclusion

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