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A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

DOI :

10.3791/50671-v

November 1st, 2013

November 1st, 2013

39,575 Views

1Department of Molecular and Biomedical Sciences, University of Maine, Orono, 2Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono

Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.

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