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Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

DOI :

10.3791/50723-v

August 16th, 2013

August 16th, 2013

10,861 Views

1Department of Immunology and Infectious Diseases, Montana State University, 2Department of Molecular Biology, Princeton University

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

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