JoVE Logo
Faculty Resource Center

Sign In

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

DOI :

10.3791/50741-v

October 22nd, 2013

October 22nd, 2013

13,623 Views

1Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine

Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.

-- Views

Related Videos

article

In vitro Biofilm Formation in an 8-well Chamber Slide

article

Invasion of Human Cells by a Bacterial Pathogen

article

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

article

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

article

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

article

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

article

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

article

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

article

A High-throughput Shigella-specific Bactericidal Assay

article

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved