JoVE Logo
Faculty Resource Center

Sign In

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

DOI :

10.3791/50867-v

9:22 min

December 13th, 2013

December 13th, 2013

10,191 Views

1Department of Nephrology, Children's Hospital of Pittsburgh of UPMC, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed by trafficking to lysosomes for degradation or to the plasma membrane for recycling. Methods described in this article are designed to study endocytosis and recycling of plasma membrane proteins.

Tags

Keywords Cell based L glutathione Protection Assay

-- Views

Related Videos

article

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

article

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

article

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

article

Crystallization of Membrane Proteins in Lipidic Mesophases

article

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

article

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

article

Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

article

Imaging Plasma Membrane Deformations With pTIRFM

article

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

article

Assays for the Degradation of Misfolded Proteins in Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved