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Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

DOI :

10.3791/55974-v

July 3rd, 2017

July 3rd, 2017

9,234 Views

1Department of Cellular and Physiological Sciences, University of British Columbia

Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.

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Cell surface Expression

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