JoVE Logo
Faculty Resource Center

Sign In

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

DOI :

10.3791/55974-v

July 3rd, 2017

July 3rd, 2017

9,225 Views

1Department of Cellular and Physiological Sciences, University of British Columbia

Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.

Tags

Cell surface Expression

-- Views

Related Videos

article

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

article

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

article

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

article

Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons

article

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

article

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

article

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

article

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

article

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

article

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved