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Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

DOI :

10.3791/50934-v

November 12th, 2013

November 12th, 2013

15,855 Views

1Australian Centre for Blood Diseases, Monash University

Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous membrane.

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