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Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

DOI :

10.3791/50950-v

December 20th, 2013

December 20th, 2013

7,064 Views

1Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität

Cellulosomes are multienzyme complexes designed for digesting cellulose. AFM-based SMFS was used to study the mechanical properties and folding configuration of cellulosome-associated protein assemblies. We present a complete workflow for protein immobilization, data acquisition, and data analysis to study the interactions of individual receptor-ligand complexes involved in cellulosome assembly.

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