December 20th, 2013
•Cellulosomes are multienzyme complexes designed for digesting cellulose. AFM-based SMFS was used to study the mechanical properties and folding configuration of cellulosome-associated protein assemblies. We present a complete workflow for protein immobilization, data acquisition, and data analysis to study the interactions of individual receptor-ligand complexes involved in cellulosome assembly.
Tags
Related Videos
Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Label-free Single Molecule Detection Using Microtoroid Optical Resonators
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved