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Sample Drift Correction Following 4D Confocal Time-lapse Imaging

DOI :

10.3791/51086-v

April 12th, 2014

April 12th, 2014

16,017 Views

1School of Biological Sciences, Monash University, 2Janelia Farm Research Campus, Howard Hughes Medical Institute

Time-lapse microscopy allows the visualization of developmental processes. Growth or drift of samples during image acquisition reduces the ability to accurately follow and measure cell movements during development. We describe the use of open source image processing software to correct for three dimensional sample drift over time.

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Sample Drift Correction

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