Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
10.5K Views
•
10:40 min
•
June 13th, 2020
DOI :
June 13th, 2020
•Teresa P. Silva1,2, Tiago G. Fernandes1, Diogo E. S. Nogueira1, Carlos A. V. Rodrigues1, Evguenia P. Bekman1,2,3, Yas Hashimura4, Sunghoon Jung4, Brian Lee4, Maria Carmo-Fonseca2, Joaquim M. S. Cabral1
1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA
Chapters in this video
0:04
Introduction
0:23
Seeding the Bioreactor
2:06
Differentiation and Maturation of Aggregates
3:26
Preparing Organoids for Cryosectioning
5:47
Immunostaining
8:27
Results: Efficient Cerebellar Differentiation and Maturation
10:09
Conclusion
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