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Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

DOI :

10.3791/51276-v

May 4th, 2014

May 4th, 2014

15,530 Views

1Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam

This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.

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Dendritic Spines

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