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Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons

DOI :

10.3791/2568-v

6:35 min

April 16th, 2011

April 16th, 2011

53,332 Views

1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda

FRAP has been used to quantify the mobility of Green Fluorescence Protein (GFP)-tagged proteins in cultured cells. We examined the mobile/immobile fractions of the GFP by analyzing the fluorescence recovery percentage after photobleaching. In this study, FRAP was performed at spines of hippocampal neurons.

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Fluorescence Recovery After Photobleaching FRAP

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