April 16th, 2014
•Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.
Tags
Related Videos
Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells
Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization
Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition
A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish
Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved