שימוש בcaspase ריבוב Assay לקביעת אפופטוזיס בדגם נייד ההיפותלמוס

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08:27 min

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April 16th, 2014

DOI :

10.3791/51305-v

April 16th, 2014

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Tammy A. Butterick¹^,², Cayla M. Duffy¹^,², Rachel E. Lee³, Charles J. Billington¹^,²^,⁴, Catherine M. Kotz¹^,², Joshua P. Nixon¹^,²

¹Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, ²Department of Food Science and Nutrition, University of Minnesota, ³Department of Integrative Biology and Physiology, University of Minnesota, ⁴Department of Medicine, University of Minnesota Medical School, University of Minnesota

Summary

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Chapters in this video

0:05

Title

2:20

Plating and Counting Cells

4:29

Determining Cell Viability and Caspase-3/7 Activity

6:34

Results: Fold Change in Caspase-3/7 Activity Normalized to Cell Viability

7:27

Conclusion

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