JoVE Logo
Faculty Resource Center

Sign In

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

DOI :

10.3791/51325-v

July 26th, 2014

July 26th, 2014

14,106 Views

1Department of Medical Biophysics, University of Toronto, 2Platform Biological Sciences, Sunnybrook Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Physical Sciences, Sunnybrook Research Institute, 5Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 6Manitoba Institute of Cell Biology, University of Manitoba

We present an image registration approach for 3-dimensional (3D) histology volume reconstruction, which facilitates the study of the changes of an organ at the level of macrostructures made up of cells . Using this approach, we studied the 3D changes between wild-type and Igfbp7-null mammary glands.

Tags

Histology

-- Views

Related Videos

article

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

article

The Ladder Rung Walking Task: A Scoring System and its Practical Application.

article

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

article

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

article

Evaluation of Mammary Gland Development and Function in Mouse Models

article

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

article

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

article

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

article

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

article

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved