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A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

DOI :

10.3791/63413-v

May 24th, 2022

May 24th, 2022

2,162 Views

1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Pathology, University of Wisconsin-Madison, 3Carbone Cancer Center, University of Wisconsin-Madison

The intravital imaging method described here utilizes collagen second harmonic generation and endogenous fluorescence from the metabolic co-factor NAD(P)H to non-invasively segment an unlabeled tumor microenvironment into tumor, stromal, and vascular compartments for in-depth analysis of 4D intravital images.

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Intravital Imaging

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