JoVE Logo
Faculty Resource Center

Sign In

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer (Video) | JoVE

DOI :

10.3791/58345-v

September 13th, 2018

September 13th, 2018

8,607 Views

1Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center, University of Michigan Medical Center, 2Department of Urology, Division of GU Oncology, Rogel Cancer Center, University of Michigan Medical Center, 3Departments of Surgery and Pathology, Perlmutter Cancer Center, NYU Langone Health

The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.

-- Views

Related Videos

article

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence (Video) | JoVE

article

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants (Video) | JoVE

article

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics (Video) | JoVE

article

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager (Video) | JoVE

article

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function (Video) | JoVE

article

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines (Video) | JoVE

article

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies (Video) | JoVE

article

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture (Video) | JoVE

article

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model (Video) | JoVE

article

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved