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08:08 min
July 9th, 2014
DOI :
10.3791/51570-v
Chapters
0:05
Title
1:24
Processing FFPE GI Tissue Blocks
3:17
DNA Extraction from Tissue Scrolls
4:30
Quantitative PCR
6:39
qPCR Results on Control Tissues
7:41
Conclusion
Summary
This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.
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