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Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

2.4K Views

09:47 min

May 10th, 2022

DOI :

10.3791/63561-v

May 10th, 2022


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Substrate Ubiquitylation

Chapters in this video

0:05

Introduction

1:08

Cell Culture

2:33

Cell Transfection

3:41

Cell Lysis and Immunoprecipitation

7:40

Results: Specific Polyubiquitylation of UXT-V2-HA Mediated by Fbxo7

9:06

Conclusion

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