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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

DOI :

10.3791/59079-v

March 23rd, 2020

March 23rd, 2020

8,932 Views

1Proteomics Center, Erasmus University Medical Center

We present a method for the purification, detection, and identification of diGly peptides that originate from ubiquitinated proteins from complex biological samples. The presented method is reproducible, robust, and outperforms published methods with respect to the level of depth of the ubiquitinome analysis.

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Keywords Protein Ubiquitination

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