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Detection of Protein Ubiquitination

42.6K Views

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09:00 min

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August 19th, 2009

DOI :

10.3791/1293-v

August 19th, 2009

•
1Signal Transduction Program, The Sanford Burnham Institute for Medical Research

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Protein Ubiquitination
Posttranslational Modification
Ubiquitin activating Enzyme E1
Ubiquitin conjugating Enzyme E2
Ubiquitin Ligase E3
Deubiquitylating Enzymes
Protein Stability
Cellular Localization
Biological Activity
Gene Mutations
Human Diseases
Cancer
Neurodegeneration
Metabolic Disorders
Pathogenesis
Detection Protocols
Cultured Cells In Vivo
Test Tubes In Vitro
Sumolyation
Neddylation

Chapters in this video

0:10

Introduction

0:42

Expression and Purification of Target Protein and Tagged Ubiquitin

3:15

Immunoprecipitation

4:51

SDS-PAGE and Immunostaining

5:48

Detection of Protein Ubiquitination in vitro

7:02

Representative Results

8:22

Conclusion

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