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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

DOI :

10.3791/51583-v

7:35 min

June 9th, 2014

June 9th, 2014

20,998 Views

1Department of Biomedical Engineering, Duke University, 2Research Triangle MRSEC, Duke University

Elastin-like polypeptides are stimulus-responsive biopolymers with applications ranging from recombinant protein purification to drug delivery. This protocol describes the purification and characterization of elastin-like polypeptides and their peptide or protein fusions from Escherichia coli using their lower critical solution temperature phase transition behavior as a simple alternative to chromatography.

Tags

Non chromatographic Purification

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