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Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

DOI :

10.3791/51652-v

October 8th, 2014

October 8th, 2014

15,805 Views

1The RH Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, 2Earth and Life Institute, Université catholique de Louvain, 3Institut des Sciences de la Vie, Université catholique de Louvain

Measuring the osmotic water permeability coefficient (Pf) of cells can help understand the regulatory mechanisms of aquaporins (AQPs). Pf determination in spherical plant cell protoplasts presented here involves protoplasts isolation and numerical analysis of their initial rate of volume change as a result of an osmotic challenge during constant bath perfusion.

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Osmotic Water Permeability Coefficient

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