High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
12.6K Views
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11:10 min
•
August 11th, 2014
DOI :
August 11th, 2014
•Jing Wu1, Roberta Pugh1, Richard C. Laughlin1, Helene Andrews-Polymenis2, Michael McClelland3, Andreas J. Bäumler4, L. Garry Adams1
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 2Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M University System Health Science Center, 3University of California, Irvine, 4Department of Medical Microbiology & Immunology, School of Medicine, University of California, Davis
Chapters in this video
0:05
Title
1:59
Murine Macrophage RAW264.7 Cell Culture
3:29
Preparation of Salmonella Wild-type and Mutants
5:19
Invasion Assay in a 96-well Culture Plate
9:58
Results: Identification of the Phenotype of Salmonella Mutants
10:46
Conclusion
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