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Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

DOI :

10.3791/64263-v

10:39 min

August 9th, 2022

August 9th, 2022

1,901 Views

1Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna (IZSLER)

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

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Automated Analysis

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