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Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

DOI :

10.3791/51806-v

August 19th, 2014

August 19th, 2014

16,697 Views

1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey

DNA repair pathways are essential for maintenance of genomic integrity and preventing mutation and cancer. The goal of this protocol is to quantify genomic instability by direct observation of chromosome aberrations in metaphase spreads from mouse B cells using fluorescent in situ hybridization (FISH) for telomeric DNA repeats.

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Chromosome Aberrations

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