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Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

DOI :

10.3791/63819-v

May 10th, 2022

May 10th, 2022

1,446 Views

1Department of Biological Sciences and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute

Here, we present a protocol for reconstituting microtubule bundles in vitro and directly quantifying the forces exerted within them using simultaneous optical trapping and total internal reflection fluorescence microscopy. This assay allows for nanoscale-level measurement of the forces and displacements generated by protein ensembles within active microtubule networks.

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