JoVE Logo

Sign In

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

31.1K Views

08:06 min

September 3rd, 2014

DOI :

10.3791/51896-v

September 3rd, 2014


Explore More Videos

Synaptic Plasma Membrane

Chapters in this video

0:05

Title

1:16

Tissue Homogenization and Fractioning

3:57

Isolating the Synaptic Plasma Membrane (SPM) Fraction

7:02

Western Blot of Fractions

7:32

Conclusion

Related Videos

article

09:49

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

10.3K Views

article

12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

11.4K Views

article

08:30

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

31.4K Views

article

05:13

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

4.0K Views

article

05:56

Polysome Profiling without Gradient Makers or Fractionation Systems

5.0K Views

article

07:55

An Improved Method to Isolate Mitochondrial Contact Sites

1.3K Views

article

14:28

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

6.1K Views

article

10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

10.4K Views

article

12:14

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

5.7K Views

article

11:09

An Easy Method for Plant Polysome Profiling

12.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved