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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

DOI :

10.3791/51960-v

7:54 min

September 2nd, 2014

September 2nd, 2014

44,984 Views

1Institute of Nutrition, Friedrich Schiller University Jena

This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization.

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Keywords Transfection

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