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Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

DOI :

10.3791/62418-v

8:11 min

August 7th, 2021

August 7th, 2021

3,506 Views

1Structural Genomics Consortium, University of Toronto, 2Department of Pharmacology and Toxicology, University of Toronto

These protocols provide the methodology used to assess the enzymatic activity of individual members of the protein arginine methyltransferase (PRMT) family in cells. Detailed guidelines on assessing PRMT activity using endogenous and exogenous biomarkers, methyl-arginine recognizing antibodies, and inhibitor tool compounds are described.

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Quantitative Methods

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