Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

23.8K Views

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07:35 min

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April 7th, 2015

DOI :

10.3791/52338-v

April 7th, 2015

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Nydia Morales-Soto¹^,², Morgen E. Anyan¹, Anne E. Mattingly¹, Chinedu S. Madukoma¹, Cameron W. Harvey³, Mark Alber³, Eric Déziel⁴, Daniel B. Kearns⁵, Joshua D. Shrout¹^,²^,⁶

¹Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, ²Eck Institute for Global Health, University of Notre Dame, ³Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, ⁴INRS-Institut Armand-Frappier, ⁵Department of Biology, Indiana University, ⁶Department of Biological Sciences, University of Notre Dame

Summary

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Keywords Bacterial Surface Motility

Liquid solid air Interface

Fluorescent bioluminescent Reporters

Time lapse Imaging

Quantification

Competing Swarming Species

Chapters in this video

0:05

Title

1:44

Media and Plate Assay Preparation

3:51

Swarm Assay Inoculation

4:51

Macroscopic Imaging of Bacterial Surface Motility

5:45

Results: Swarming Dynamics of Various Bacteria Strains

6:47

Conclusion

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