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Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

DOI :

10.3791/62180-v

February 4th, 2021

February 4th, 2021

5,026 Views

1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, 2Department of Physiology, Perelman School of Medicine, University of Pennsylvania

Presented here is a procedure to express and purify myosin 5a followed by a discussion of its characterization, using both ensemble and single molecule in vitro fluorescence microscopy-based assays, and how these methods can be modified for the characterization of nonmuscle myosin 2b.

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