February 22nd, 2015
•Here we describe an efficient and versatile protocol to induce, monitor and analyze novel glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate and generate tumors with histo-pathological characteristics of human GBM.
Tags
Related Videos
Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
Image-guided Convection-enhanced Delivery into Agarose Gel Models of the Brain
Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.
Mouse Kidney Transplantation: Models of Allograft Rejection
A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy
Surgical Models of Gastroesophageal Reflux with Mice
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved