Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
13.1K Views
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16:01 min
•
January 26th, 2015
DOI :
January 26th, 2015
•1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University
Chapters in this video
0:05
Title
2:37
Transfection of Rat Basophilic Leukemia (RBL) Cells
6:06
Measuring NPY mRFP Exocytosis
7:48
Time-lapse Microscopy of Exocytosis
10:51
Image Analyses
13:00
Results: Quantifying Mast Cells Secretory Granule (SG) Size and their Exocytosis
14:44
Conclusion
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