Sign In

JoVE Journal

Neuroscience

This content is Open Access.

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

10.4K Views

09:05 min

May 6th, 2015

May 6th, 2015

10,426 Views

0:05

Title

1:59

Eye Enucleation and Isolation of Retina

3:22

Slice Preparation

4:59

Two-photon Microscopy

6:14

Light Stimulation and Recording

6:53

Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors

8:10

Conclusion

We describe a protocol to monitor Ca2+ dynamics in the axon terminals of cone photoreceptors using an ex-vivo slice preparation of the mouse retina. This protocol allows comprehensive studies of cone Ca2+ signaling in an important mammalian model system, the mouse.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More