March 19th, 2015
•Biolistic transformation is a method used to generate stable integration of DNA into the genome of the opportunistic pathogen Cryptococcus neoformans through homologous recombination. We will demonstrate biolistic transformation of a construct, which has the gene encoding acetate kinase fused to the fluorescent tag mCherry into C. neoformans.
Tags
Related Videos
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved