June 13th, 2021
•This article presents a small-scale plasma membrane isolation protocol for the characterization of Candida albicans ABC (ATP-binding cassette) protein Cdr1, overexpressed in Saccharomyces cerevisiae. A protease-cleavable C-terminal mGFPHis double tag with a 16-residue linker between Cdr1 and the tag was designed to facilitate the purification and detergent-screening of Cdr1.
Tags
Related Videos
Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
Imaging Plasma Membrane Deformations With pTIRFM
Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Sample Preparation for Mass Cytometry Analysis
Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved