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JoVE Journal

Medicine

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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

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07:36 min

May 1st, 2015

May 1st, 2015

14,195 Views

0:05

Title

1:14

Foreskin and Lymphatic Malformation Cell Suspension Preparation

4:01

Antibody Staining of Lymphatic Endothelial Cell (LEC) Surface Markers

5:49

Results: Representative Foreskin and Lymphatic Malformation LECs

6:53

Conclusion

The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.

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