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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

DOI :

10.3791/52564-v

April 8th, 2016

April 8th, 2016

14,168 Views

1Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Centre, 2Department of Dermatology, Radboud University Medical Centre

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

Tags

Lymphocyte Isolation

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