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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

DOI :

10.3791/52830-v

June 5th, 2015

June 5th, 2015

17,742 Views

1School for Mental Health and Neuroscience, Maastricht University, 2Department of Neurosciences, Institut d'Investigació Germans Trias i Pujol

Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.

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Recombinant Human IgG Monoclonal Antibodies

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